دراسة استجابة بعض هجن البندورة المحمية إزاء العدوى الاصطناعية بفيروس التجعد البني على ثمار البندورة ToBRFV

Abstract

In this study during 2021-2022 season, fourteen imported hybrids of greenhouse tomato were tested for their response to artificial inoculation by mechanical means with the viral isolate MZ447794 of Tomato brown rugose fruit virus (ToBRFV), which had been previously characterized biologically, serologically, and molecularly and is submitted in the GenBank. All the tested hybrids exhibited susceptibility to the studied virus, displaying symptoms that varied among hybrids and generally included: mild to severe leaf mosaic, variable leaf deformations including narrow leaves and crinkles, in addition to discoloration and brown rugose on the fruits. The occurrence of infection with the studied virus was confirmed in all plants of the tested inoculated hybrids using DAS-ELISA assay.

The tested and virus inoculated hybrids differed in the number of days post inoculation  required for symptoms expression, which ranged between 14 and 24 days. The percentage loss in yield caused by artificial infection with the studied virus was calculated for all tested tomato hybrids and ranged overall from 8.55% to 49.49%, compared to the healthy, non-inoculated control of each hybrid. The yield loss percentage was ranged between 19.17% and 34.96% in 9 out of 14 tested hybrids. The lowest percentage loss in yield was observed in Almasa F1 and Yolia F1 hybrids amounting 8.55% and 9.18% respectively.