Study of the Factors Influencing the Biodegradation of Petroleum Compounds Using Bacteria Isolated from the Local Environment

Abstract

This study focused on the effectiveness testing of a mixture of bacteria isolated from the local environment to study their efficiency in reducing Petroleum compounds with engine oil as an example. The effect of processing conditions on the effectiveness of the selected bacteria was studied. Highly efficient bioremediation models were obtained in which the optimal values of the studied variables (temperature, pH, treatment time, oil compound concentration) were determined for each bacterial isolate, based on the Response Surface Methodology (RSM) methodology The mixture bacteria Klebsiella pneumoniae،Bacillus subtilis  ،and Bacillus cereus achieved a reduction efficiency at the optimal values of the experimental conditions: temperature (31.32)˚C, pH(7.6), oil compound concentration (174.09) mg/l, treatment time (17.8) days.