A study of the effect of extracts from the leaves of the plants Inula viscosa L. and Artemisia annua L. on some types of bacteria causing infectious diseases in poultry

Abstract

 Due to the increasing rate of bacterial resistance to commercial antibiotics, it became necessary to resort to using medicinal and aromatic plants because of their great diversity and richness in bioactive compounds. The plants *Elephanthemum* and *Artemisia* were used, as they are known for their effectiveness against bacteria causing infectious diseases. This research aimed to study the effectiveness of five extracts from *Elephanthemum* and *Artemisia* against bacteria causing hemorrhagic enteritis in poultry. This study was conducted in the Microbiology Laboratory at the Faculty of Agricultural Engineering, Latakia University. Leaves of *Elephanthemum* and *Artemisia* were collected in 2025, then dried and stored until use. Five extracts from the leaves of *Elephanthemum* and *Artemisia* were used: chloroform, diethyl ether, ethanol, and an aqueous extract at concentrations of 60 microliters. Their effect was studied on two types of bacteria causing enteritis in poultry: *Pseudomonas aeruginosa* and *Clostridium perfringens*. Sensitivity tests were conducted on the studied bacteria using the previous extracts, and the study showed that Pseudomonas aeruginosa and Clostridium perfringens bacteria were sensitive to the five extracts used. The use of the ethanol extract of thyme and wormwood at a concentration of 60 microliters gave the highest value in the average diameter of the bacterial growth inhibition ring for the bacteria used, Clostridium perfringens and Pseudomonas aeruginosa. The diameters of bacterial growth inhibition in wormwood plants were recorded as (27.20 and 24.16 mm), followed by the diameters of bacterial growth inhibition in thyme plants as (26.91 and 21.01 mm), respectively, compared to the DMSO 5% control.The results indicate that extracts from the leaves of elecampane and wormwood plants have antimicrobial activity against pathogenic bacterial species that cause septic infections, and thus could be a source of natural antibiotics in the future.