تقييم فعالية المستخلص المائي لقشور ثمار الرمان ضد البكتيريا Streptomyces scabies المسببة لمرض الجرب الشائع على البطاطا

Abstract

This research aimed to isolate and identify the causal disease agent, which causes common scab disease on potato tubers. After isolating and growing the pathogenic bacteria from infected potato tubers from different areas in Lattakia Governorate, Syria. The pathogenic bacterial isolates were grown on nutrient agar (NA) medium, and the cultural and morphological characteristics of the bacterial colonies were studied, and some biochemical tests and pathogencity tests were conducted. The effect of the aqueous extract of pomegranate fruit peels on the growth of the pathogenic bacteria was also tested in the laboratory on the nutrient medium using the disc diffusion method, which depends on placing paper discs with specific concentrations of the extract on the surface of the nutrient medium (NA) on which the bacterial isolate was spread and incubated at a temperature at 28 C for 48 hours. thereafter that, the diameter of the inhibition zone around the paper disc treated with the extract at its different concentrations was measured and the percentage of inhibition of the pathogenic bacteria was measured. The study of the cultural characteristics of the studied bacteria, biochemical tests and pathogencity test showed that the bacteria causing the disease, Streptomyces scabies, is the causal agent of common scab disease. The results showed that the aqueous extract of pomegranate fruit peels at different concentrations (10, 15, 25)% showed an inhibitory effect on the growth of the bacteria Streptomyces scabies, where the inhibition rate reached (17.44, 19.22, 30.78) % for the three concentrations used of pomegranate fruit peels extract, respectively. The results of this research show that pomegranate fruit peels extract is a biobacterial pesticide that can be used in the future to combat the bacteria causing common scab disease.