نمذجة وأمثلة عملية استخلاص المواد الفينولية ومضادات الأكسدة من نبات Cyperus esculentus باستخدام منهجية سطح الاستجابة (RSM)

Abstract

Cyperus esculentus (Tiger nut) is one of the most widespread herbal plants in the Mediterranean basin, and its extracts are a rich source of bioactive components that have many beneficial properties for health. This study aims to make the extracts of this plant more effective by improving the extraction process of phenolics and antioxidants from C. esculentus using response surface methodology (RSM) based on the design (BBD). Three independent variables of the process were studied, namely extraction time (X1), extraction temperature (X2) and solid-liquid ratio (X3), at three levels (1-, 0, 1+), through (15) experimental points, and their effects on two responses (namely antioxidant capacity (Y1:DPPHsc%) and phenolic content (Y2:TPC)) were evaluated. The data were analyzed and simulation models for the extraction process were built in the form of a second-order polynomial using DOE/RSM/BBD. The adequacy of the models was proven using analysis of variance (ANOVA). The significant effects of factors and their interactions on extraction efficiency were studied at 95% confidence interval. Desirability approach was used to identify and evaluate the best conditions for the extraction process that give maximum yield of (TPC) and (DPPHsc). RSM indicated that the best values ​​for (X1), (X2) and (X2) with a desirability value equal to (D = 99.3%) which indicates the optimal solution, were (25.9 h), ((45.7ºC and (0.21 g/mL) respectively, which give the best predictive values ​​for (Y1) and (Y2) under the optimal conditions which are (63.92%) and mgGAE /100 g dw 178.54) respectively.