دراسة مخبرية لإمكانية استخدام عزلات محلية من الفطرBeauveria bassiana (Bals.) Vuil. في تثبيط نمو الفطر الممرض للبندورة Fusarium oxysporum f.sp. lycopersici
Keywords:
Antagonism, Entomopathogenic Fungi, vascular wilt, Dual planting, spore suspension, Beauveria bassiana.Abstract
This research was conducted to evaluate, in the laboratory, the ability of two local isolates of the entomopathogenic fungus Beauveria bassiana (B2 and B4) to inhibit the growth of two isolates of the phytopathogenic fungus Fusarium oxysporum f. sp. lycopersici (FO6 and FO7). Different methods were followed to assay the antagonism, dual planting in the same plate on the same day, and dual planting by planting B. bassiana isolates before two days of planting F. oxysporum isolates at the same plate. In addition, the fungus B. bassiana was applied using the spore suspension in several concentrations (105, 106, 107, and 108 spore/ ml).
Results demonstrated the ability of the used isolates of the fungus B. bassiana to inhibit the growth of the vascular wilt fungus F. oxysporum f. sp. lycopersici in all followed methods with significantly superior of the method of planting B. bassiana isolates two days of F. oxysporum isolates planting and the spore suspension method by the highest concentrations (107 and 108 spore suspension). Inhibition rates of isolate B2 reached 71.92 and 71.46 % after 10 days of treatment against the growth of FO6 and FO7, respectively.